Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 28;20:15330338211045831. doi: 10.1177/15330338211045831

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 1. — Downregulation of CBX2 inhibited glioma cell proliferation, invasion, and self-renewal. (A) The data (UALCAN, http://ualcan.path.uab.edu/index.html) showed that the expression of CBX2 was higher than that in normal brain tissue (P < .001). (B) The level of CBX2 was higher in glioma tissues. And with the increase of glioma grade, the expression of CBX2 increased, the expression of CBX2 in Grade IV glioma tissues was the highest. (C and D) The expression of CBX2 was analyzed in glioma cell lines by RT-PCR and western blotting. (E and F) CBX2-shRNA transfection efficiencies were verified by RT-PCR and western blotting (P < .001). (G and H) CCK-8 and colony formation assays demonstrated that CBX2-shRNA-mediated knockdown of CBX2 decreased the proliferation of glioma cells (P < .01). (I) Transwell assays showed that CBX2 downregulation reduced invasion compared to that in the scramble group. (J) Sphere formation analysis showed that the downregulation of CBX2 decreased the glioma stem cell frequencies (P < .05). (K) Knockdown of CBX2 reduced the levels of N-cadherin, slug, and snail and increased the level of E-cadherin.

Abbreviations: CBX, ChromoBox; CCK-8, cell counting kit-8; qRT-PCR, quantitative real-time PCR.