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. 2021 Oct 28;218(12):e20192070. doi: 10.1084/jem.20192070

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© 2021 Isringhausen et al.

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Figure 3. — Durable functional impairment of CARc after chronic LCMV-cl13 infection. (A–D) Expression of key genes involved in HSC maintenance measured by RT-qPCR in sorted BM CARcs of uninfected control (ctrl) or 7 and 56 dpi with LCMV-cl13. Expression of Cxcl12 (A), Scf (B), Vcam (C), and Il-7 (D) are shown as percentage of HRPT expression (n = 3–5 samples from at least two independent experiments). (E–H) Concentrations of CXCL12 (E) and SCF (F) in BM extracts from one hind leg (femur and tibia) at different time points after LCMV-cl13 infection as measured by ELISA. (G) Principal component (PC) analysis plot for RNA-seq analyses of CARcs from uninfected control mice and 56 dpi. (H) GSEA graph for REACTOME gene sets overrepresented and underrepresented in CARcs isolated from the BM of mice 56 dpi compared with uninfected control BM. The net enrichment score (NES) and false discovery rate (FDR) for different gene sets are represented in the graph. Immunoreg. interac. bet., immunoregulation interaction between. (I) Integrated dot plot/heatmaps depicting expression levels from RNA-seq analysis of individual genes from selected REACTOME gene sets in H that are significantly up- or down-regulated in CARc 56 dpi compared with uninfected controls. (J and K) Concentrations of IFNα and IFNγ in BM extracts from one hind leg (femur and tibia) at different time points after LCMV-cl13 infection as measured by ELISA (n = 5 mice from at least two independent experiments). Statistical significance was analyzed by two-tailed Mann–Whitney U test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, P > 0.05..