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. 2021 Oct 28;218(12):e20192070. doi: 10.1084/jem.20192070

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© 2021 Isringhausen et al.

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Figure S1. — Effects of chronic infections in BM hematopoietic and HSC function. (A) Representative image showing maximum-intensity projection of femoral diaphyseal regions of BM from uninfected control (ctrl) or 7 dpi with LCMV-cl13. Blue, CD105 (BM sinusoids); white, Ter119 (erythroid progenitors). Scale bars, 500 µm (left); 50 µm (right). (B) Gating scheme used to phenotypically define HSC and HSPC populations in the BM of control (CTRL) and infected mice. FSC, forward scatter; SSC, side scatter. (C) Analysis showing CD45.1 donor engraftment (black circle, scale on left y axis) and donor lineage distribution in T cell (green), B cell (blue), and granulocyte (red) compartments as percentage of total CD45.1 engraftment (scale on right y axis). Mice were considered engrafted when the percentage of CD45.1⁺ cells in PB was >0.5% 4 mo after transplantation in all three lineages. Empty columns indicate total CD45.1 donor engraftment <0.5%. (D and E) Representative histograms and quantification of the fraction of cleaved caspase 3⁺ HSCs in uninfected mice and 3 and 7 dpi. Statistical significance was analyzed by Mann–Whitney U test. *, P < 0.05; ***, P < 0.001.