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. 2021 Oct 18;64(20):15250–15261. doi: 10.1021/acs.jmedchem.1c01286

Figure 2.

Figure 2

Synthesis of [Gd-DOTA]4-MI. (i) Fmoc-Lys(Fmoc)-OH, PyBOP, DIPEA, DMF, 1 h, 20% acetic anhydride; (ii) 40% piperidine-DMF (5 min) and 20% piperidine-DMF (15 min); (iii) Fmoc-Lys(Fmoc)-OH, PyBOP, DIPEA, DMF,1 h; (iv) 40% piperidine-DMF (5 min) and 20% piperidine-DMF (15 min); (v) DOTA(tBu)3CONH(CH2)5COOH, PyBOP, DIPEA, DMF, 2 h ; (vi) TFA, DCM, TIS 49:49:2 (20 min) and TFA 100% (12 h); (vii) GdCl3 in water; and (viii) N-succinimidyl-3-maleimidopropionate in 50 mM phosphate buffer.