Figure 4. . Combination of quercetin and 2-methoxyestradiol inhibited epithelial–mesenchymal transition in PC-3 cells by downregulating the expression of mesenchymal markers and upregulation of epithelial markers.

Effect of all the three different groups of drugs on the mRNA expression of EMT markers. (A) E-cadherin. (B) N-cadherin. (C) Vimentin. Gene mRNA levels were normalized with β-actin and expressed as fold change with respect to the control group. Data represented as mean ± SEM from three independent experiments. The statistical analysis was performed using ANOVA with Tukey’s post hoc multicomparison test (*p < 0.05; **p < 0.01; ***p < 0.001).

2-ME: 2-methoxyestradiol; EMT: Epithelial–mesenchymal transition; Qu: Quercetin.