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. 2021 Sep 24;7(9):FSO747. doi: 10.2144/fsoa-2021-0028

Figure 5. . Quercetin and 2-methoxyestradiol downregulated Wnt signaling components of PC-3 cells at both gene and protein levels.

Figure 5. 

Effect of all the three different groups of drugs on the mRNA expression of Wnt components: (A) β-catenin and (B) cyclin D1. Gene mRNA levels were normalized with β-actin and expressed as fold change with respect to the control group. Data represented as mean ± SEM from three independent experiments. The statistical analysis was performed using ANOVA with Tukey’s post hoc multicomparison test (*p < 0.05; **p < 0.01; ***p < 0.001). (C) The expression of Wnt signaling proteins viz., cyclin D1 and β-catenin after treatment with different drug combinations was analyzed by western blotting. L1: Control; L2: TGF-β (20 ng/ml); L3: Quercetin (20 μM); L4: Quercetin (20 μM) + TGF-β (20 ng/ml); L5: 2-ME (10 μM); L6: 2-ME (10 μM) + TGF-β (20 ng/ml); L7: Quercetin (20 μM) + 2-ME (10 μM) + TGF-β (20 ng/ml); L8: Quercetin (20 μM) + 2-ME (10 μM).

2-ME: 2-methoxyestradiol; EMT: Epithelial–mesenchymal transition; Qu: Quercetin.