Table 3.
Biological pathways obtained after GO enrichment of common genes between RNA-seq and ChIRP-seq datasets. Detail description of genes under each pathway is given below
| Gene | Fold change in expression (from RNAseq dataset) | Distance to TSS (from ChIRP-seq dataset) | Biological function |
|---|---|---|---|
| Signal Transduction (GO:0007165, p.val: 0.005062523) | |||
| TG | 2.56054 | -734 | hormone activity and carboxylic ester hydrolase activity. |
| FGD1 | 1.63956 | -319 | Activates CDC42. Plays a role in regulating the actin cytoskeleton and cell shape. |
| ANK2 | 1.69588 | -3310 | protein kinase binding and structural constituent of cytoskeleton |
| PDE1B | −1.77852 | -1499 | calmodulin binding and 3',5'-cyclic-AMP phosphodiesterase activity |
| NRXN2 | 1.51534 | -2035 | transmembrane signaling receptor activity and calcium channel regulator activity |
| NRXN3 | −2.08903 | -661 | cell adhesion molecule binding |
| PDGFRB | −3.39665 | -162 | regulation of many biological processes including embryonic development, angiogenesis, cell proliferation and differentiation. |
| CHN1 | −2.19144 | -194 | GTPase activator activity and ephrin receptor binding |
| PTCH2 | 4.70794 | -7374 | hedgehog receptor activity and hedgehog family protein binding. |
| HPGDS | −1.58043 | -1303 | calcium ion binding and magnesium ion binding |
| Neurotransmitter secretion (GO:0007269, p. val:0.009331) | |||
| NRXN2 | 1.51534 | -2035 | transmembrane signaling receptor activity and calcium channel regulator activity |
| NRXN3 | −2.08903 | -661 | cell adhesion molecule binding |
| DGKI | −5.02254 | 8365 | NAD+ kinase activity and diacylglycerol kinase activity |
| Cytoskeleton organization (GO:0007010, p.val: 0.010913659) | |||
| FGD1 | 1.63956 | -319 | Activates CDC42. Plays a role in regulating the actin cytoskeleton and cell shape. |
| ANK2 | 1.69588 | -3310 | protein kinase binding and structural constituent of cytoskeleton |
| WTIP | −2.31319 | -7255 | involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration |
| CTNNA2 | 1.67243 | -1990 | structural molecule activity and structural constituent of cytoskeleton |
| Positive regulation of phospholipase C activity (GO:0010863, p. val: 0.025440324) | |||
| PDGFRB | −3.39665 | -162 | regulation of many biological processes including embryonic development, angiogenesis, cell proliferation and differentiation. |
| PTAFR | −2.05699 | -3443 | G protein-coupled receptor activity and lipopolysaccharide binding |
| Vocalization behavior (GO:0071625, p.val:0.039299) | |||
| NRXN2 | 1.51534 | -2035 | transmembrane signaling receptor activity and calcium channel regulator activity |
| NRXN3 | −2.08903 | -661 | cell adhesion molecule binding |
| Cell differentiation (GO:0030154, p.val: 0.042536642) | |||
| IGSF10 | 1.70255 | -5900 | control of early migration of neurons expressing gonadotropin-releasing hormone |
| CPLX2 | −1.76176 | -9666 | Negatively regulates the formation of synaptic vesicle. Positively regulates a late step in exocytosis of various cytoplasmic vesicles |
| TDRD6 | −2.02059 | -4622 | involved in germ cell development |
| TP53 | −1.89693 | -148 | Acts as a tumour suppressor in many tumour types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. |
| ZIC1 | −4.16215 | 1079 | transcriptional activator. Involved in neurogenesis |
| Neuron cell-cell adhesion (GO:0007158, p. val:0.044788079) | |||
| NRXN2 | 1.51534 | -2035 | transmembrane signaling receptor activity and calcium channel regulator activity |
| NRXN3 | −2.08903 | -661 | cell adhesion molecule binding |
| Negative regulation of smoothened signaling pathway (GO:0045879, p.val:0.05296437) | |||
| SERPINE2 | −2.36671 | -3485 | signaling receptor binding and serine-type endopeptidase inhibitor activity. |
| PTCH2 | 4.70794 | -7374 | hedgehog receptor activity and hedgehog family protein binding. |
| Regulation of small GTPase mediated signal transduction (GO:0051056, p.val:0.056180682) | |||
| FGD1 | 1.63956 | -319 | Activates CDC42. Plays a role in regulating the actin cytoskeleton and cell shape. |
| CHN1 | −2.19144 | -194 | GTPase activator activity and ephrin receptor binding |
| ARHGAP29 | −2.32394 | -1824 | GTPase activator activity and PDZ domain binding |