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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Cell Stem Cell. 2021 May 12;28(7):1233–1247.e4. doi: 10.1016/j.stem.2021.02.022

Figure 6. Testing the requirement of limbal stem cells for corneal homeostasis and wound healing.

Figure 6.

(A) Experimental strategy to test the requirement of stem cells in the inner limbus in sustaining the homeostatic maintenance of their corneal-fated progeny. (B) Diagram depicting a high magnification view of the limbus before and after ablating inner limbal clones (red). Outer limbal clones are shown in blue, intact inner limbal clones are shown in black. (C) Regression of corneal lineages after ablation of their respective stem cells in the inner limbus. Example shows global and high-magnification live views of the eye and limbus, imaged at the indicated time points, before and after the ablation of stem cells in the inner limbus. A pseudo-colored overlay is used to demarcate labeled clones in the outer limbus (blue), as well as the ablated (red) and intact (black) clones in the cornea. Also see Movie S7. (D) Imaging time course after corneal epithelial debridement wound. Yellow arrow indicates cells in the outer limbus entering the cornea. Panels C and D show tiled images of the cornea and limbus constructed from multiple fields-of-view. Scale bars: 20 μm.