TABLE 2.

Categories of regulons affected by knockdown of MLL3

Module	Category	H3K4me1	ERα	Direction of Effect	Overlap	P‐value	Bonferroni (α < 0.003125)	Jaccard Index	Odds Ratio
1	1	Loss ‐ 1356	Loss ‐ 494	Upreg	416	2.90E‐227	Yes	0.3	29.7
	2	Loss ‐ 1356	Gain ‐ 159	Upreg	107	1.50E‐38	Yes	0.1	8.7
2	3	Gain ‐ 1150	Gain ‐ 333	Downreg	151	8.90E‐35	Yes	0.1	4.4
	4	Gain ‐ 1150	Loss ‐ 1170	Downreg	658	3.40E‐266	Yes	0.4	13.1
3	5	Loss ‐ 0	Loss ‐ 1170	Downreg	0	1	No	0	0
4	6	Loss ‐ 0	Gain ‐ 333	Downreg	0	1	No	0	0
5	7	Gain ‐ 53	Loss ‐ 494	Upreg	8	0.039	No	0	2.2
6	8	Gain ‐ 53	Gain ‐ 159	Upreg	7	2.30E‐04	Yes	0	6.5

The table displays the organization of ZR751 differentially expressed genes (DEG) based on whether a gain or loss of associated ERα and H3K4me1 peaks were observed in the MLL3 KD compared to the control. The background size used for the one‐sided Fisher's exact test was 6677 genes, as this was the number of DEG to which the peaks were matched. The GeneOverlap R package, by Li Shen was utilized. Categories in bold had significant overlaps. Significant categories sharing two characteristic changes were collapsed into modules.