FIGURE 4.

Exercise reduced doxorubicin (Dox)‐induced oxidative stress after 2 weeks in peripheral blood mononuclear cells (PBMCs) from immunodeficient and immunocompetent mice. (A) Reactive oxygen species production in PBMCs. Nude mice were treated with phosphate‐buffered saline (black line), Dox alone (red line), exercise alone (green line), or Dox + exercise (blue line). Peripheral blood was collected 24 h after therapy and PBMCs were isolated. Dihydroethidium staining was used for the measurement of intracellular superoxide using flow cytometry. Mean fluorescent intensity for dihydroethidium is shown for representative mice. (B) Balb/c mice were treated with phosphate‐buffered saline (black line), Dox alone (red line), exercise alone (green line), or Dox + exercise (blue line). Peripheral blood was collected 24 h after therapy. PBMCs were then isolated and stained for intracellular peroxides by flow cytometry using dichlorofluorescein. Mean fluorescent intensity is shown for representative mice