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. 2021 Mar 27;49(12):e67. doi: 10.1093/nar/gkab199

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Comparison of RNA quantitation methods for k-Seq. Total RNA amount quantified for samples incubated with different BYO concentrations, determined by spike-in method vs. direct quantification using Qubit or qPCR, correlates well (Pearson's r = 0.999, P-value = ) and with comparable relative standard deviation (Supplementary Figure S2). Error bars show standard deviations calculated from triplicates for reacted samples.

Inline graphic — Comparison of RNA quantitation methods for k-Seq. Total RNA amount quantified for samples incubated with different BYO concentrations, determined by spike-in method vs. direct quantification using Qubit or qPCR, correlates well (Pearson's r = 0.999, P-value = ) and with comparable relative standard deviation (Supplementary Figure S2). Error bars show standard deviations calculated from triplicates for reacted samples.