Table 1.
Coverage of local sequence space in the variant pool containing four ribozyme families. N/A = not applicable. s.d. = standard deviation. The calculation of expected counts in the unreacted pool does not include effects of sequencing error
Order of mutants ( ) |
# of unique sequences | # of analyzable sequences | Fraction of possible unique sequences that were analyzable | Mean counts in the unreacted pool (s.d.) | Expected counts in the unreacted pool |
|---|---|---|---|---|---|
| 0 | 4 | 4 | 1.000 | 1 243 500 (151 356) | 1 136 125 |
| 1 | 252 | 252 | 1.000 | 37 599.9 (10 607.1) | 37 455 |
| 2 | 7560 | 7560 | 1.000 | 1198.3 (454.8) | 1234 |
| 3 | 143 640 | 143 482 | 0.999 | 39.7 (18.9) | 40.7 |
| 4 | 1 939 140 | 590 115 | 0.304 | 1.7 (1.3) | 1.34 |
| ≥5 | N/A | 23 343 | N/A | 0.7 (0.5) | N/A |