Table 1. Bacterial strains and plasmids used in this study.
| Bacterial strain or plasmid | Locus | Relevant characteristics | Reference or source |
|---|---|---|---|
| E. coli strain | |||
| DH5α | F − λ − φ80dLacZΔM15Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK − mK+) supE44 thi-1gyrA relA1 |
Takara, Kyoto, Japan | |
| S17-1 | Thi pro hsdR-hsdM + recA (chr::RP4-2-Tc::Km::Tn7) | Schäfer et al. (1994) | |
| P. savastanoi pv. glycinea | |||
| Psg | Psg wild-type | MAFF301684 | |
| VTB8 | PsgB076_27735 | cfa6::mTn5, Nalr, Kmr | This study |
| VTC23 | PsgB076_27795 | cmaA::mTn5, Nalr, Kmr | This study |
| VTD16 | PsgB076_27730 | cfa5::mTn5, Nalr, Kmr | This study |
| VTD28 | PsgB076_27725 | cfa4::mTn5, Nalr, Kmr | This study |
| VTD29 | PsgB076_27740 | cfa7::mTn5, Nalr, Kmr | This study |
| VTD30 | PsgB076_27720 | cfa3::mTn5, Nalr, Kmr | This study |
| VTE13 | PsgB076_27750 | cfa8::mTn5, Nalr, Kmr | This study |
| VTE17 | PsgB076_27740 | cfa7::mTn5, Nalr, Kmr | This study |
| VTF3 | PsgB076_27705 | cfa2::mTn5, Nalr, Kmr | This study |
| VTG22 | PsgB076_27735 | cfa6::mTn5, Nalr, Kmr | This study |
| VTG29 | PsgB076_27740 | cfa7::mTn5, Nalr, Kmr | This study |
| VTG41 | PsgB076_27725 | cfa5::mTn5, Nalr, Kmr | This study |
| VTH39 | PsgB076_27735 | cfa6::mTn5, Nalr, Kmr | This study |
| VTI15 | PsgB076_27740 | cfa7::mTn5, Nalr, Kmr | This study |
| VTR4 | PsgB076_27740 | cfa7::mTn5, Nalr, Kmr | This study |
| VTT14 | PsgB076_27805 | cmaC::mTn5, Nalr, Kmr | This study |
| VTT22 | PsgB076_27730 | cfa5::mTn5, Nalr, Kmr | This study |
| VTB2 | PsgB076_28870 | Type III effector protein XopAD::mTn5, Nalr, Kmr | This study |
| VTB52 | PsgB076_09885 | Heat shock protein 90 (Hsp90)::mTn5, Nalr, Kmr | This study |
| VTH40 | PsgB076_15537 | MFS transporter::mTn5, Nalr, Kmr | This study |
| VTM22 | PsgB076_27425 | Hypothetical protein::mTn5, Nalr, Kmr | This study |
| VTO15 | PsgB076_01129 | ABC transporter permease::mTn5, Nalr, Kmr | This study |
| VTO41 | PsgB076_06035 | Sigma factor algU::mTn5, Nalr, Kmr | This study |
| VTP20 | PsgB076_26940 | DNA-binding protein::mTn5, Nalr, Kmr | This study |
| VTE41 | PsgB076_09720 | Unknown, Nalr, Kmr | This study |
| VTF6 | PsgB076_10315 | Unknown, Nalr, Kmr | This study |
| VTO37 | PsgB076_19532 | Unknown, Nalr, Kmr | This study |
| VTT8 | PsgB076_26618 | Unknown, Nalr, Kmr | This study |
|
algU mutant (VTO41) + pDSKG-algU |
algU mutant complemented with pDSKG-algU, Nalr, Genr |
This study | |
| Plasmid | |||
| pBSLC1 | Transposon vector constructed by ligation of pBSL118 and pHSG396 at EcoRI site, Ampr, Kmr, Cmr | Sawada et al. (2018) | |
| pDSK519 | Broad-host-range cloning vector, Kenr | Keen et al. (1988) | |
| pDSKG | Broad-host-range cloning vector, Genr | This study | |
| pDSKG-algU | The vector containing algU gene inserted into pDSKG, Genr | This study | |
Note:
Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Genr, gentamicin; Kmr, kanamycin resistance; Nalr, nalidixic acid resistance.