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. 2021 Nov 1;35(21-22):1461–1474. doi: 10.1101/gad.348780.121

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© 2021 Shao et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — ZFP423 recruitment of the NuRD complex is required for its suppression of thermogenic gene expression in mature adipocytes. (A) Schematic representation of 30 zinc finger (ZF) motifs within murine ZFP423 with highlighted location of the N-terminal NuRD-interacting domain (NID) and C-terminal EBF binding domain. (B) Western blot of the indicated protein expression in whole-cell lysates (top) and ZFP423 immunoprecipitates (bottom) from differentiated transduced immortalized brown stromal vascular cells expressing EBF2 and either FLAG-tagged (FL) ZFP423^ZF1-30 or ZFP423^ΔNID. (C) Relative mRNA levels of the indicated thermogenic genes in differentiated immortalized brown stromal vascular cells expressing EBF2 and either FLAG-tagged (FL) ZFP423^ZF1-30 or ZFP423^ΔNID. Bars represent mean + SEM. (*) P < 0.05 by one-way ANOVA. n = 3 biological replicates.