Figure 1.

T-CM decreases tumorigenesis and migration of HepG2 cells. (A) BALB/c nude male mice were injected subcutaneously at the right back with HepG2 cells grown in T-CM or DMEM. After 9 days, tumor size was measured (n=3). (B) Tumors were isolated, chopped and seeded onto 100-mm cell culture plates 5 days after injection (n=3). Photographs were taken on a microscope 2 (upper) and 9 days (lower) after culture. Original magnification, ×100. (C) Number of cell clusters on the cell culture plate was counted (n=3/mouse). (D) In vitro scratch wound healing assay was conducted. HepG2 cells were cultured in the presence or absence of T-CM and the migrated cells over the scratched area were observed after 24 and 48 h. Magnification, ×40. (E) Wound closure area was calculated and compared. A total of >10 experiments were analyzed, and each experiment was repeated three times. Data were analyzed by paired t-test. ^*P<0.05 vs. control. (F) Wound width was measured and distance was calculated (n=10). Data are presented as the mean ± SEM and were analyzed by one-way ANOVA with multiple comparison by Sidak test. ^*P<0.05 and ^***P<0.001. T-CM, tonsil-derived mesenchymal stem cell conditioned medium.