Table 1.
The effect of mediator on the ability of sLac and DyP2 to modify native lignin present in aspen wood powder.a
| L/(L+PS) | P/NP | G/Ar | S/Ar | |
|---|---|---|---|---|
| H2O2 | 0.590±0.006 | 1.23±0.03 | 0.64±0.02 | 0.68±0.02 |
| Mn2+ | 0.599±0.004 | 1.25±0.05 | 0.64±0.04 | 0.69±0.03 |
| ABTS | 0.587±0.012 | 1.24±0.04 | 0.66±0.02 | 0.69±0.04 |
| ABTS+H2O2 | 0.588±0.015 | 1.29±0.04 | 0.68±0.01 | 0.73±0.04 |
| sLac+ABTS | 0.580±0.011 | 1.12±0.04*** | 0.48±0.02*** | 0.48±0.02*** |
| DyP2+H2O2 | 0.583±0.006 | 1.23±0.03 | 0.61±0.02 | 0.67±0.02 |
| DyP2+H2O2+ABTS | 0.582±0.010 | 1.18±0.05 | 0.59±0.02 | 0.63±0.04 |
| DyP2+H2O2+Mn2+ | 0.566±0.009** | 1.16±0.04 | 0.55±0.03*** | 0.62±0.03* |
a
Lignin modification metrics are indicated and defined in the main text. All reactions contained powdered aspen. Reported values are based on six ToF-SIMS spectra (n=6). ANOVA analysis with Tukey’s post-test: *p<0.05; **p<0.005; ***p<0.0005 compared to the no-enzyme controls, including aspen wood powder alone. Refer to Figure 2 for the conditions of aspen, aspen+sLac, and aspen+DyP2.