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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Biometals. 2021 Jan 3;34(2):291–301. doi: 10.1007/s10534-020-00279-5

Figure 1. Marginal zinc deficiency deceased zinc status and further increased whole blood LPS-induced IL6 response in old mice.

Figure 1.

Young (2 mo) and old (24 mo) C57BL/6 mice were fed a zinc adequate (ZA) diet or marginally zinc deficient (MZD) diet for 6 wks. Serum zinc levels were measured by ICP-OES at the end of the study (A). Whole blood were collected at the beginning (wk 0) (B) and end of study (wk 6) (C) to determine LPS-induced IL6 response after 24h ex vivo LPS stimulation (10 ng/ml). Data represent mean ± SEM (n = 7–10 per treatment group). Two-way ANOVA was used to test for main effects of zinc status and age and their interaction, followed by Tukey’s multiple comparisons test where appropriate. ** P<0.01, NS = not significant.