Fig 5. Limited contribution of alternative homolog conjunction to meiotic chromosome missegregation in condensin II protein mutants.
(A-C) The disappearance of the alternative homolog conjunction protein UNO during M I was analyzed by time-lapse imaging in (A) control (+ / +) and (B) Cap-D3 mutants (Cap-D3EY00456/ Df). Beyond UNO-EGFP, spermatocytes expressed His2Av-mRFP, and in case of the Cap-D3 mutants also Cenp-A/Cid-EGFP. The prominent UNO-EGFP dot on the chrXY pairing site (arrowheads) disappeared during anaphase I with comparable rapid kinetics in both genotypes, as confirmed (C) by quantification of the UNO-EGFP dot intensity over time. (D) Progression into and through M I was analyzed by time-lapse imaging in Cap-D3 mutants (Cap-D3EY00456/ Df) (top) and Cap-D3 mnm double mutants (Cap-D3EY00456/ Df; mnmz3-5578/ mnmz3-3298) (bottom). Chromosomes condense into a single clump irrespective of presence or absence of AHC. Time (min:sec) indicated relative to the onset of NEBD I. Scale bars = 3 μm.