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. 2021 Oct 25;2021:2255017. doi: 10.1155/2021/2255017

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Copyright © 2021 Chien-Ming Chu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

CD4 lymphocytes were identified with positive CD3 and CD4. The percentage of CD4 lymphocytes in stimulated peripheral blood mononuclear cells (PBMCs) with recombinant human high-mobility group box 1 (rhHMGB1) in a patient was 1.96%. Cell death was identified with positive 7-aminoactinomycin D (AAD) and annexin V. The percentage of cell death in CD4 lymphocytes was 1.00%. CD8 lymphocytes were identified with positive CD3 and CD8. The percentage of CD8 lymphocytes in rhHMGB1-stimulated PBMCs in a patient was 0.99%. The percentage of cell death in CD8 lymphocytes was 9.96%. Monocytes were identified with positive CD11b and CD14. The percentage of monocytes in rhHMGB1-stimulated PBMCs in a patient was 0.58%. The percentages of cell death in monocytes were 12.98%.