Figure 5.

RNA-seq assay of mouse hepatocytes between Man treatment and NAFLD model groups. (a) The Pearson correlation between samples. (b) Differentially expressed gene volcano map (∣log₂ (fold change)∣>1 and padj ≤ 0.05). (c) Differentially expressed genes clustering heat map of 1601 (∣log₂ (fold change)∣>1 and padj ≤ 0.05). (d, e) The Gene Ontology (GO) biological process enrichment of upregulation (d) or downregulation (e) of differentially expressed genes, and the threshold value for significant enrichment of GO function was padj ≤ 0.05. (f, g) One of the representations of KEGG pathway (f) about upregulation of differentially expressed genes and its ID, description, p value, and padj value (g). (h, i) One of the representations of KEGG pathway (h) about downregulation of differentially expressed genes and its ID, description, p value, and padj value (i). (j) The NLRP3/Casp-1/GSDMD-mediated inflammatory signal pathway in (h), which is closely related to the effects of Man treatment.