Figure 9.

The effects of Man on NLRP3 inflammasome signal axis and its inhibition of NLRP3-mediated pyroptosis in vitro. (a) After serum starvation treatment, cells were untreated or treated with PA-BSA or PA-BSA+Man or PA-BSA+Met as indicated for 24 h. Then, total cell proteins were made; p-AMPKα (Thr172) (p-AMPKα), NLRP3, caspase-1, caspase-1 (P10), GSDMD-N, IL-1β, and β-actin were detected by western blot. Representative blot pictures as shown. The protein levels of p-AMPKα (b), GSDMD-N (c), and NLRP3/caspase-1 (p10)/IL-β (d) were normalized to β-actin, respectively, and plotted as fold of the DMSO+10% BSA-treated group. Values are represented as the mean ± SD (n = 3). ^∗∗∗p < 0.001 vs. DMSO+10% BSA and ^##p < 0.01 and ^###p < 0.001 vs. DMSO+PA-BSA.