Skip to main content
. 2021 Oct 25;2021:8293699. doi: 10.1155/2021/8293699

Figure 8.

Figure 8

HucMSCs+ucPRP promote the proliferation of ovarian granulosa cells and reduce apoptosis. (a) Immunohistochemical examination of the expression of the granulosa cell proliferation marker PCNA and the apoptosis marker caspase-3 and TUNEL staining examination of granulosa cell apoptosis. (b) Caspase-3 mainly located in the ovarian granulosa cells. H score analysis of caspase-3, a marker of granulosa cell apoptosis. Scale bar: 100 μm, 25um. (c) PCNA mainly located in the ovarian granulosa cells. H score analysis of the granular cell proliferation marker PCNA. Scale bar: 100 μm, 25 μm. (d) TUNEL staining to detect the apoptosis of ovarian granulosa cells. Scale bar: 100 μm, 25 μm. Green represents apoptotic cells, and blue represents cell nuclei. Data represent the mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.