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Sequences of PCR primers and length of products.
| Gene | Sequence of primer (5′-3′) | Length of product |
|---|---|---|
| GADPH | Forward: GTGGACCTGACCTGCCGTCTAG | 149 |
| Reverse: GAGTGGGTGTCGCTGTTGAAGTC | ||
| Oct-4 | Forward: GTGGTCCGAGTGTGGTTCTGTAAC | 90 |
| Reverse: CCCAGCAGCCTCAAAATCCTCTC | ||
| Nanog | Forward: GATGCAAGAACTCTCCAACATC | 178 |
| Reverse: CTGGTGGTAGGAAGAGTAAAGG | ||
| SOX2 | Forward: CAGCATGTCCTACTCGCAGCAG | 117 |
| Reverse: CTGGAGTGGGAGGAAGAGGTAACC |
