Figure 4.

Insulin induced temporal changes in fatty acids and lipid mediators in early- and late-fasted seal pups (n = 5 animals/period). The 72 metabolites were condensed into 12 cluster components using variable clustering in JMP Pro 15.1, an implementation of the principal components analysis-based VARCLUS procedure for SAS. The cluster components were clustered into 3 groups of 4 (A–D, E–H, I–L) using K-nearest neighbors. Clusters are named by representative suites of metabolites contained within each cluster. Contrast posttests were used to evaluate mean differences between early and late groups at each time, as well as differences across time. Differences detected between early and late fast are indicated at P <0.05 with an asterisk (*). Differences detected across time in comparison to T0 are indicated at P <0.05 with a triangle (▴).