Figure 4.

Triglyceride phenotypes after chronic high-fat feeding. At the conclusion of 6 mo of either normal chow diet (NCD) or high-fat diet (HFD) Angptl4^LivKO and Angptl4^fl/fl mice (n = 5–9 mice/group) were fasted (6 h) and injected intravenously with ³H-triglyceride containing chylomicrons. A: clearance of radiolabel from the plasma 1, 5, 10, and 15 min after injection. Points represent percentage of radiolabel remaining in the plasma at the indicated time points compared with the 1-min time point (means ± SE). B: uptake of radiolabel (% injected dose/g tissue) into the indicated tissues 15 min after injection of ³H-chylomicrons (means ± SE). C: heart, quadriceps muscle (Quad), epididymal adipose tissue (eWAT), subcutaneous adipose tissue (sWAT), and brown adipose (BAT) tissue from fasted (6 h) male Angptl4^LivKO and Angptl4^fl/fl mice (n = 5–13/group) were harvested and lipase activity was measured. D and E: liver was harvested from fasted (6 h) male Angptl4^LivKO and Angptl4^fl/fl mice [NCD groups (D) and HFD groups (E)] (n = 6–7/group). To distinguish between hepatic versus lipoprotein lipase activity, lipase activity was measured in the presence or absence of 1 M NaCl. Bars show relative lipase activity in each tissue normalized to Angptl4^fl/fl (means ± SE). F: fasted (6 h) mRNA expression of Lpl in heart, liver, quadriceps muscle (quad), epididymal white adipose tissue (eWAT), subcutaneous white adipose tissue (sWAT), and brown adipose tissues (BAT) of male Angptl4^LivKO and Angptl4^fl/fl mice fed either a normal chow diet (NCD) or a high-fat diet (HFD; 60% by kcal) for 6 mo (means ± SE; n = 5–8/group). #P < 0.05, ##P < 0.01, ###P < 0.001 for dietary differences by two-way ANOVA. HL, hepatic lipase; LPL, lipoprotein lipase.