Figure 5.

Glucose tolerance and insulin sensitivity of Angptl4^LivKO mice. A and B: glucose tolerance tests were performed on fasted (6 h) male Angptl4^LivKO and Angptl4^fl/fl mice after either 11 wk (A) or 24 wk (B) of either a normal chow diet (NCD) or high-fat diet (HFD). Mice were injected with glucose (2 g/kg for NCD; 1.3 g/kg HFD) and blood glucose concentrations were measured over 2 h. Points represent glucose levels (means ± SE; n = 7–11 mice) at each respective time point. Bar graphs represent area under the curve (means ± SE) for all time points. *P < 0.05 by repeated measures ANOVA. C and D: insulin tolerance tests were performed on fasted (4 h) male an Angptl4^LivKO mice after either 12 wk (C) or 25 wk (D) of either a normal chow diet (NCD) or high-fat diet (HFD). Mice were injected with 0.75 U/mL of human insulin (Humalin-R) and blood glucose concentrations were measured over 90 min. Points represent glucose levels (means ± SE; n = 7–11) at each respective time point. Bar graphs represent area under the curve (means ± SE) for all time points. #P < 0.05, ###P < 0.001 for dietary differences by two-way ANOVA. *P < 0.05, for individual genotype-specific differences by repeated measures ANOVA.