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. 2021 Aug 16;321(4):E464–E478. doi: 10.1152/ajpendo.00144.2021

Figure 7.

Figure 7.

Plasma measures and lipase activity in chronic HFD-fed Angptl4LivKO mice after overnight fast. Following 6 mo of either NCD or HFD, Angptl4fl/fl and Angptl4LivKO mice were fasted for 18 h and plasma triglyceride levels (A), plasma NEFA levels (B), and liver triglyceride content (C) were assessed (means ± SE; n = 7–9 mice/group). D: heart, quadriceps muscle (Quad), epididymal adipose tissue (eWAT), subcutaneous adipose tissue (sWAT), and brown adipose (BAT) tissue from fasted (18 h) male Angptl4LivKO and Angptl4fl/fl mice (n = 7–9/group) were harvested and lipase activity was measured. E and F: liver was harvested from fasted (18 h) male Angptl4LivKO and Angptl4fl/fl mice [NCD groups (E) and HFD groups (F)] mice (n = 6–9/group). To distinguish between hepatic versus LPL activity, lipase activity was measured in the presence or absence of 1 M NaCl. Bars show relative lipase activity in each tissue normalized to Angptl4fl/fl (means ± SE). #P < 0.05, ##P < 0.01, ###P < 0.001 for dietary differences by two-way ANOVA. **P < 0.01 for individual genotype-specific differences by multiple comparisons after two-way ANOVA (Tukey’s correction). HFD, high-fat diet; LPL, lipoprotein lipase; NCD, normal chow diet; HL, hepatic lipase; NEFA, nonesterified fatty acid.