Table 3.
Preliminary protein isolation data
| Buffer | Tissue | Homogenization Technique |
|||||
|---|---|---|---|---|---|---|---|
| Glass Homogenizer | Plastic Tube and Pestle | PowerGen | Glass Tube and Pestle | Omni Hard Tissue Probe | Omni Soft Tissue Probe* | ||
| House | Muscle | NA | 80 | 48 | 89 | 116 | NA |
| Adipose | NA | 1.5 | 2.3 | 1.4 | 2.3 | NA | |
| RIPA* | Muscle | 125 | 103 | 246 | 156 | 151 | 187 |
| Adipose | 2.6 | 3.2 | 2.9 | 2.6 | 3.4 | 3.5 | |
| Minute Protein | Muscle | NA | NA | NA | NA | NA | NA |
| Extraction Column | Adipose | NA | 1.9 | NA | NA | NA | NA |
All units are in (µg protein·mg tissue−1). NA, method pairing was not attempted. *Final methods used for this investigation were the Omni Soft Tissue Probe for homogenization and radioimmunoprecipitation assay (RIPA) buffer for protein extraction.