Figure 7.

Effects of Hsp70 on surface expression of Mer and ADAM17 maturation in alveolar macrophages following mechanical ventilation. Tlr4^+/+ and Tlr4^−/− mice were injected intravenously with 10 μg recombinant human Hsp70 or vehicle and then mechanically ventilated with a high tidal volume (20 mL/kg) for 4 h. Bronchoalveolar lavage (BAL) fluid was collected, and alveolar macrophages were isolated from ventilated lungs on day 2. The plasma membrane fraction was extracted, and protein expression was determined by Western blot analysis. A: effects of Hsp70 on surface expression of Mer on alveolar macrophages and soluble Mer (sMer) in BAL fluid after ventilation. Top: representative blots showing surface expression of Mer on alveolar macrophages and soluble Mer (sMer) in BAL fluid. Bottom: protein quantification (normalized to Na⁺/K⁺-ATPase) by densitometry. B: effects of Hsp70 on immature proform a disintegrin and metalloprotease 17 (pADAM17) and mature ADAM17 (mADAM17) in alveolar macrophages. Left: representative blots showing protein expression of pADAM17 and mADAM17. Right: protein quantification (normalized to GAPDH) by densitometry. n = 6 lungs (from 3 male and 3 female mice). *P < 0.05, **P < 0.01, one-way ANOVA with Bonferroni post hoc test. Data represent means ± SD. TLR4, Toll-like receptor 4.