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. 2021 Aug 16;321(4):F443–F454. doi: 10.1152/ajprenal.00205.2021

Figure 1.

Figure 1.

Several specialized proresolving mediator receptors are expressed in the rat bladder, and the tissue expression patterns of some change during bladder outlet obstruction (BOO). All images show immunostaining of bladder sections (5 µm) using rabbit primary antibodies. The first column shows sections from control rats stained with a rabbit isotype control antibody. The second column shows sections from control bladders but stained with antibodies to the indicated receptors (low mag). The third column shows higher magnitude (high mag) micrographs from the area indicated by the box in the appropriate image from the second column. The fourth column shows sections from bladders taken from 12-day BOO rats and stained for the indicated receptors. The fifth column shows higher magnitude micrographs from the area indicated by the box in the appropriate picture from the fourth column. Brackets indicate the urothelium. In CC and DD, arrows indicate individual cells positive for G protein-coupled receptor (GPR)18. Slides were developed using a Vectastain ABC-horseradish peroxidase (HRP) [HRP_ kit (PK-4001), Vector Laboratories]. All parameters for the slides used in this figure (incubation times, HRP product development times, etc.) were identical between all samples and for each antibody. All slides specifically used in this figure were stained on the same day, and identical antibody dilutions were used between control and BOO samples. Micrographs were taken with identical settings (exposure times, light intensity, etc.). Scale bar = 50 µm for the first, second, and fourth columns (low mag) and 20 µm for the third and fifth columns (high mag). All staining was repeated a minimum of three times to ensure consistency. The primary antibodies were as follows: isotype control (NBP2-36463; Novus, 1:100; A, F, K, P, U, and Z), formyl-peptide receptor-2 (FPR-2; NLS1878, Novus, 1:100; B–E), leukotriene B4 receptor 2 (BLT-1; LS-A1494, LS-Bio, 1:100; G–H), chemerin receptor 23 (ChemR23; LS-B12924, LS-Bio, 1:100; L–O), GPR37 (ab218134, Abcam, 1:200; Q–T), leucine-rich repeat-containing G protein-coupled receptor 6 (LGR6; ab126747, Abcam, 1:100; V–Y), and GPR18 (ab150618, Abcam, 1:250; AA–DD).