Figure 3.

Ac2-26 inhibits NLR family pyrin domain containing 3 (NLRP3) activation. Primary urothelial cells were incubated in 96-well plates overnight, washed, and given Ac2-26 for 1 h at the indicated doses. NLRP3 was then activated with 0.625 mM ATP for 1 h. Caspase-1 activity was assessed by the cleavage of DEVD-AFC as described in materials and methods. Results were normalized to ATP-only controls and are presented as means ± SE; n = 8, 8, 7, 8, 7, 8, 8, 7, 8, 7, 8, and 8, respectively. **P < 0.01 and ***P < 0.001 by ANOVA and a Student-Newman-Keuls test.