Figure 5.

Ac2-26 (Ac) suppresses bladder inflammation during bladder outlet obstruction (BOO) and enhances resolution after deobstruction (Deobs). Inflammation was assessed by Evan’s blue dye extravasation. Dye (25 mg/kg) was injected intravenously, and 1 h later bladders were removed, weighed, and placed in 1 mL formamide overnight (56°C). Absorbance (620 nm) was measured, and the results were calculated from a standard curve. Data are presented as means ± SE; n = 7, 7, 27, 9, 14, and 21, respectively. *P < 0.05, **P < 0.01, and ***P < 0.001 by ANOVA and a Student-Newman-Keuls test. Con, control.