Figure 9.

Mast cell inhibition during CP1-induced lower urinary tract symptoms attenuates myosin light chain-2 (MLC2) phosphorylation in the prostates of mice. Whole prostates from control, CP1-infected mice (untreated), and CP1-infected cromolyn sodium salt + cetirizine di-hydrochloride (CrS + CeHCl)-treated mice at 14 days (“early”) and 35 days (“late”) post CP1 infection were lysed in 1× RIPA lysis buffer. A: whole cell lysates were examined by Western blot analyses using the indicated antibodies, and representative Western blots for each group of mice at the early time point are shown. B: quantifications of the Western blot data shown in A (n = 8 for early and n = 6 for late). Data are plotted as arbitrary values obtained by normalizing the band intensity for phosphorylated (p)MLC2 to the band intensities of total MLC2 and then normalizing this to the band intensity of GAPDH, and data are expressed as fold changes over control. Values are presented as means ± SE. Each dot represents an individual mouse. *P < 0.05 (one-way ANOVA with Fisher’s least significant difference test). ns, not significant.