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. 2021 Aug 16;321(4):F431–F442. doi: 10.1152/ajprenal.00171.2021

Figure 3.

Figure 3.

Global cyclophilin D (CypD) knockout (KO) (gKO) suppresses proximal tubule cell cycle arrest during unilateral ureteral obstruction (UUO). Wild-type (WT) and global CypD KO mice were subjected to UUO or sham operation for 7 days. A: paraffin-embedded kidney sections were used to carry out immunofluorescent staining for evaluating the amount of Ki-67-positive cell proliferation (green) (n = 5 or 6). Lotus tetragonolobus lectin (LTL; red) was used for a marker of proximal tubule. Ki-67-positive proliferating cells and phospho-histone H3 (p-histone H3)-positive cell cycle-arrested cells were evaluated from 5 randomly chosen fields per kidney (n = 5 or 6). DAPI (blue) was used for counterstaining. B: expression levels of cyclin B1 and D1 were examined by Western blot analysis (n = 5), and representative Western blots are shown. Anti-GAPDH antibody was used as a loading control. Expression levels were evaluated using ImageJ software. C: number of phospho-histone H3 (green)-positive cells as a marker of G2/M arrest were counted in the same method as that of Ki-67. Scale bars = 50 µm. Data are expressed as means ± SD. *P < 0.05 vs. sham. #P < 0.05 vs. WT UUO.