Figure 8.

Proximal tubule-specific cyclophilin D knockout (PT-CypD KO) alleviates kidney inflammation during unilateral ureteral obstruction (UUO). Wild-type (WT) control and PT-CypD KO (PT-KO) mice were subjected to UUO or sham operation for 7 days. A and C: paraffin-embedded kidney sections were used to carry out immunohistochemistry to evaluate the number of polymorphonuclear leukocyte (PMN)- and F4/80-positive cells (n = 5 or 6). Hematoxylin stain (blue color) was used for counterstaining. Arrowheads indicate PMN- or F4/80-positive cells. B and D: numbers of PMN-positive neutrophils (B; brown color) and F/80-positive macrophages (D; brown color) were counted from 5 randomly chosen fields per kidney (n = 5 or 6). Expression levels were evaluated using ImageJ software. Scale bars = 50 µm. Data are expressed as means ± SD. *P < 0.05 vs. sham; #P < 0.05 vs. WT UUO.