Fig. 2.

Entinostat suppresses oxazolone-induced contact hypersensitivity and increases IL-10⁺ regulatory B cells in vivo. (A) Flow cytometry analysis of splenic IL-10⁺ B cells and (B) CD1d^hiCD5⁺ B Breg subsets in CHS mice with or without entinostat treatment were performed. (C) The ear thickness of CHS mice was measured daily for 4 days after the challenge with oxazolone. The ear epidermal thickness (D) and representative images (E) of ear tissues are shown after being stained with H&E (scale bar, 100 μm). All values are presented as the mean ± SEM or representative images (E) from three independent experiments (n = 4 per group for each experiment). *P < 0.05, **P < 0.01, n.s., not significant by Student’s t-test. OXZ, oxazolone. Ent, entinostat. ACE, acetone.