Fig. 4.

Entinostat enhances the binding of NF-κB at the proximal promoter region for IL-10 expression. Splenic B cells stimulated by LPS for 1 h. Chromatin immunoprecipitation assay was performed with anti-HDAC1 or anti-NF-κB p65 antibody, and respective proteins binding at the hyper sensitive site, p50 binding site, and proximal promoter regions for IL-10 expression were analyzed by real-time PCR. (A, B) Graphs show the relative binding amount of HDAC1 and NF-κB to three promoter regions. (C) Splenic B cells stimulated by LPS with or without 200 nM of entinostat for 1 h. Chromatin immunoprecipitation assay was performed with anti-HDAC1 or anti-NF-κB p65 antibody at the proximal promoter region as in panel A. The relative expression data are the mean ± SEM from at least three independent experiments. *P < 0.05. n.s., not significant by Student’s t-test. Ent, entinostat.