FIG. 11.

hPRA does not associate with the ERα transcription complex. HeLa cells were transiently transfected with 500 ng of 5XGal4-TATA-LUC, 50 ng of pBKC-βgal, 1,000 ng of pM-hPRA, and either 1,000 ng of pVP16-T (control), pVP16-hPRA, or pVP16-ERα (gray bars). Transcriptional activity was assayed on the 5XGal4-TATA-LUC reporter following the addition of 10⁻⁷ M 17-β-estradiol or 10⁻⁷ M R5020 and represents an indirect measure of the binding of the fusion proteins. Transfections were normalized for efficiency as mentioned above. The data are represented as fold induction over the control interaction between Gal4-hPRA and VP16-T in the absence of ligands, which was normalized to 1.0 (black bars). Each data point represents the average of triplicate determinations from three separate experiments.