FIG. 8.
The deacetylase inhibitor TSA can partially reverse hPRA-mediated repression of hER transcriptional activity. HeLa cells were transiently transfected with 1.5 μg of 3XERE-TATA-LUC, 50 ng of pBKC-βgal, 500 ng of pRST7-ERα, and either 481 ng of pBKC-hPRA or 467 ng of pBKC-Rev-TUP1 (not shown). Variable amounts of pBSII-KS were used for a total of 3 μg of DNA. Transcriptional activity of the 3XERE-TATA-LUC reporter was measured 24 h after the addition of 10−7 M 17-β-estradiol and 10−7 M RU486, alone or in combination with increasing concentrations of TSA (0, 10−8, 10−7, and 10−6 M). A control was done in the absence of ligands (not shown). The data are presented as percent activation where 100% represents a measure of 17-β-estradiol-dependent transactivation by hER in the absence of RU486 (CONT). The data from one representative experiment are shown (n = 2). The average coefficient of variation at each point was <10%.