Fig. 5.

eRAPA adjunct treatment with moxifloxacin reduces the number of necrotic lesions that develop and infiltrative pulmonary pathology. (A) H&E images of lung tissues from Mtb-infected C3HeB/FeJ mice that received either moxifloxacin with Eudragit or moxifloxacin and eRAPA from week 7 to week 9 post infection were scanned using a Leica SCN-400 F whole-slide scanner up to 40× magnification. Images were captured at various magnifications using Aperio ImageScope to show histopathological differences between the two groups. The presence of necrotic granulomas is indicated with blue asterisks. (B) Inflamed lung area was calculated using ImageJ. (C) Differences in pulmonary pathology (Ci,Cii) and thickening of lung interstitium (Ciii,Civ) between the two groups are shown using representative images at 200 µm (10×) and 70 µm (30×) magnification. Data are means±s.e. **P<0.005 (unpaired, two-tailed Student's t-test). Five mice were included in each group. Scale bars: 5 mm (A); 200 µm (C).