Table 1.
LXRα peptides detected by S-trap column coupled with mass spectrometry (MS)
| Sample | Total identified peptides | −10IgP [LXRα] | Coverage LXRα |
Supporting peptides [LXRα] | Unique | Amino acids position |
|
|---|---|---|---|---|---|---|---|
| Peptides | Start | End | |||||
| Tumor 2 Rep 1 | 3500 | 52.32 | 13% | Q.AQ(+.98)GGSSC(+57.02)ILR.E | No | 32 | 41 |
| A.QGGSSC(+57.02)ILR.E | No | 33 | 41 | ||||
| R.MPHSAGGTAGV.G | No | 46 | 56 | ||||
| R.ASSPPQ(+.98)ILPQ.L | No | 196 | 205 | ||||
| R.ASSPPQILPQLSPEQ(+.98)LGMIEK.L | No | 196 | 216 | ||||
| R.AMN(+.98)ELQLN(+.98)DAEFALLI.A | No | 345 | 360 | ||||
| Tumor 2 Rep 2 |
3971 | 58.66 | 22% | K.C(+57.02)RQ(+.98)AGM(+15.99)REEC(+57.02)VLSEEQ(+.98)IRLK.K | No | 158 | 177 |
| R.QEEEQAHATSLPPR.A | No | 182 | 195 | ||||
| R.ASSPPQILPQLSPEQ(+.98)LGMIEK.L | No | 196 | 216 | ||||
| L.SPEQ(+.98)LGMIEK.L | No | 207 | 216 | ||||
| G.M(+15.99)IEKLVAAQQ(+.98)Q(+.98).C | No | 213 | 223 | ||||
| K.QLPGFLQLSR.E | No | 274 | 283 | ||||
| Q.VEFINPIFEFSR.A | No | 333 | 344 | ||||
| R.AMNELQLNDAEFALLI.A | No | 345 | 360 | ||||
| Tumor 5 Rep 1 |
3713 | 61.52 [α4 +LXRα homolog] | 19% | MQ(+.98)QTSWN(+.98)PLGGTC(+57.02)K.Q | Yes [α4] | −14 | −1 |
| 59.30 [LXRα homolog] | 16% | R.AEPPSEPTEIRPQ(+.98)K.R | No | 70 | 83 | ||
| KC (+57.02)RQAGMR.E | No | 196 | 208 | ||||
| R.ASSPPQILPQLSP.E | No | 196 | 208 | ||||
| R.ASSPPQILPQLSPEQ(+.98)LGMIEK.L | No | 196 | 216 | ||||
| G.MIEKLVAAQQQC(+57.02)NRR.S | No | 213 | 227 | ||||
| K.TSAIEVMLLETSRRYN(+.98)PG.S | No | 292 | 309 | ||||
| Tumor 5 Rep 2 |
3412 | 38.73 | 9% | C.VLSEEQIR.L | No | 168 | 175 |
| R.ASSPPQILPQLSPEQ(+.98)LGMIEK.L | No | 196 | 216 | ||||
| R.ASSPPQ(+.98)ILPQ.L | No | 196 | 205 | ||||
| R.EDQIALLK.T | No | 284 | 291 | ||||
Amino acids position numbers based on LXRα1 structure. The gray highlight indicated unique peptides of LXR variant detected by MS.