REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
LXRα	R&D System	Cat. PP-PPZ0412-00; RRID:AB_2154888
LXRβ	Active Motif	Cat. 61177; RRID:AB_2614980
HPRT	Santa Cruz	Cat. Sc-376938; RRID: n/a
IRDye 800CV goat anti-mouse	LI-COR Biosciences	Cat. 926-68170; RRID:AB_10956589
IRDye 680RD goat anti-rabbit	LI-COR Biosciences	Cat. 926-68071; RRID:AB_10956166
Isotype IgG2a	Cell Signalling	Cat. 61656S; RRID:AB_2799613
Biological samples
Frozen tumor samples (n=38), see Table S3	Leeds Breast Research Tissue Bank	Ethic approval ID: 15/HY/0025 and LBTB_TAC_1/17
Chemicals, peptides, and recombinant proteins
Dulbecco’s Modified Eagle Medium (DMEM)	Thermo Fisher	Cat. 31966047
Fetal Bovine Serum (FBS)	Thermo Fisher	Cat. 11560636
Lipofectamine RNAiMAX	Thermo Fisher	Cat. 13778030
OptiMeM	Thermo Fisher	Cat. 31985062
bis(sulfosuccinimidyl)suberate	Thermo Fisher	Cat. A39266
GW3965	Tocris	Cat. 2474
Dynabeads Protein A	Thermo Fisher	Cat. 100002D
PMSF protease and phosphatase inhibitor	Thermo Fisher	Cat. 78440
NUPAGE LDS sample loading buffer	Thermo Fisher	Cat. NP0007
DTT reducing agent	Thermo Fisher	Cat. NP0004
TBS blocking buffer	Li-COR	Cat. 927-60001
Critical commercial assays
ReliaPrepTM RNA Cell Miniprep System	Promega	Cat. Z6012
Cytoplasmic & Nuclear RNA Purification Kit	Norgen	Cat. 21000
GoScriptTM Reverse Transcription kit	Promega	Cat. A5003
BCA kit	Thermo Fisher	Cat. 23227
Experimental models: Cell lines
HEPG2	ATCC	HB-8065
MCF7	ATCC	HTB-22
BT474	ATCC	HTB-20
MDA.MB.231	ATCC	CRM-HTB-26
MDA.MB.468	ATCC	HTB-132
MDA.MB.453	ATCC	HTB-131
MDA.MB.157	ATCC	HTB-24
Oligonucleotides
CONTROL siRNA	Origene	Cat. SR30004
siLXRα	Origene	Cat. SR322981
siLXRβ	Origene	Cat. SR305039
Primers for qPCR, see Table S4	This paper	N/A
Software and algorithms
Clustal Omega-Multiple sequence alignment	EMBL-EBI	https://www.ebi.ac.uk/Tools/msa/clustalo/
PeptideMass tool	Swiss Institute of Bioinformatics	http://www.expasy.org/tools/peptide-mass.html
Diagram Venn	Bioinformatics & Evolutionary Genomics	http://bioinformatics.psb.ugent.be/webtools/Venn/
GraphPad Prism v8	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Image Studio™ Lite	LI-COR Biosciences	https://www.licor.com/bio/image-studio/
ApE-A plasmid Editor	M.Wayne Davis	https://jorgensen.biology.utah.edu/wayned/ape/
AutoCAD	Autodesk	https://www.autodesk.co.uk
Other
NCBI	National Center for Biotechnology Information	https://www.ncbi.nlm.nih.gov
ENSEMBL	European Bioinformatics Institute (EMBL-EBI)	https://www.ensembl.org/index.html
UNIPROT	EMBL-EBI, Swiss Institute of Bioinformatics (SIB), and Protein Information Resources (PIR)	https://www.uniprot.org
cBioportal	Memorial Sloan Kettering Cancer Center	https://www.cbioportal.org
TCGA Splicing Database (TSVdb)	Key Laboratory of Disease Proteomics of Zhejiang Province	http://www.tsvdb.com