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. 2021 Oct 19;11:684965. doi: 10.3389/fcimb.2021.684965

Table 4.

Results of rapid metagenomics, clinical diagnosis and medical records of patients with typical fastidious pathogens, S. pneumoniae, H. influenzae and M. catarrhalis.

Age | Sex | Sample type a Clinical department b Type of infection c Comorbidities | Complication d Empiric therapy |Switched therapy e PCT f (ng/mL) CRP g (mg/L) Outcome | LOS h (Days) c Rapid metagenomics result Comments i
(Patients with S. pneumoniae identified by rapid metagenomics and urine antigen test)
57 | F | B RICU SCAP None | Sepsis; RF LVF+CRO+IPM | LVF; PIP/TAZ 5.48 372 Recovered | 23 S. pneumoniae (98.7%) Early de-escalation would be achieved when clinicians knew Meta, as good as urinary antigen.
33 | M | B RICU SCAP None | ARDS; RF MFX 8.49 44.5 Recovered | 8 R. mucilaginosa (21.5%); S. pneumoniae (10.8%)
44 | M | B Ward CAP-ICH NS; Diabetes; Influenza | None PIP/TAZ+MFX | AMC 0.58 11 Recovered | 9 S. pneumoniae (96.8%)
(Patients with S. pneumoniae identified by rapid metagenomics and culture)
70 | M | S Ward HAP
(late-onset)
CTEPH; Chronic pulmonary heart disease | Cardiogenic shock FOX | CSL 0.26 21 Dead | 15 L. rhamnosus (24.5%); S. pneumoniae (12.3%);
S. parasanguinis(11.0%); R. dentocariosa(10.6%)
Narrow spectrum antibiotics could be chosen when S. pneumoniae was the most likely pathogen from Meta of’ sputum.
82 | M | S Ward CAP Tuberculous pleurisy; GERD | None LVF 0.28 19 Recovered | 11 S. parasanguinis (18.8%); S. salivarius (18.3%);
R. mucilaginosa(15.4%); S. pneumoniae (6.7%)
78 | M | S Ward AECOPD II None None / 5 Recovered | 3 C. argentoratense (24.2%); S. mitis (20.0%);
S. pneumoniae (12.3%)
(Patients with S. pneumoniae identified by rapid metagenomics and qPCR)
84 | M | B RICU SCAP COPD; Chronic pulmonary heart disease; GERD | Sepsis; RF CSL+AZM | CSL; LVF; VCM 5.36 39.1 Dead | 30 S. pneumoniae (58.3%) De-escalation was not achieved because the clinicians did not know the causative pathogens.
75 | M | S Ward HAP
(early-onset)
Cerebral infarction; Mental disorders | None ETP 0.17 59 Recovered | 10 S. pneumoniae (64.3%)
77 | F | S Ward AEBX None CSL 0.55 177 Recovered | 14 S. mitis (55.5%); S. pneumoniae (10.5%);
P. aeruginosa (1.3%)
56 | M | B RICU SCAP Influenza | RF; ARDS PIP/TAZ+LVF 9.89 38.07 Recovered | 19 S. pneumoniae (78.7%); C. striatum (6.7%)
71 | M | B Ward Lung abscess Metastatic carcinoma of lung; Colorectal cancer | None CSL | PIP/TAZ 0.13 70 Recovered | 23 S. pneumoniae (80.0%)
65 | M | S Ward AECOPD II Lung cancer; GERD | None None / 24 Recovered | 29 R. mucilaginosa (16.9%);
S. pseudopneumoniae (12.1%); S. pneumoniae (6%)
52 | F | S Ward AEBX GERD | None PIP/TAZ 0.29 6 Recovered | 7 P. aeruginosa (53.5%); S. mitis (19.0%);
S. parasanguinis (6.4%); S. pneumoniae (5.2%)
(Patients with S. pneumoniae identified by rapid metagenomics only)
59 | M | B Ward Lung abscess Diabetes | None PIP/TAZ+ORN 0.29 58 Recovered | 49 P. aeruginosa (24.3%); S. mitis (26.2%);
P. micra(15.9%); S. pneumoniae (5.6%)
Non-verification might be low-abundance of S. pneumoniae.
88 | M | S Ward CAP-ICH NHL; Chemotherapy; GERD; Lacunar infarction | Sepsis MOX | MOX; LVF; MEPM; VCM 1.15 47 Recovered | 32 S. mitis (56.1%); S. pneumoniae (11.3%)
69 | F | S Ward CAP-ICH Multiple Myeloma; GERD | Cardiac failure; Pleural effusion MOX | PIP/TAZ; LVF; AMK; TGC 0.69 23.5 Recovered | 21 R. mucilaginosa (27.2%); L. pentosus (14.2%);
V. parvula(14.0%); E. faecium (3.1%);
S. pneumoniae (1.7%)
(Patients with H. influenzae identified by rapid metagenomics and culture)
70 | F | B Ward CAP Bronchiectasis | None CRO / 12 Recovered | 12 H. influenzae (93.2%) Narrower spectrum antibiotics should be used.
74 | F | B Ward HAP
(early-onset)
Sjögren’s syndrome; ILD | None INH | INH; MFX 0.4 85 Recovered | 19 H. influenzae (88.4%); M. intracellulare (8.1%)
65 | F | B Ward AEBX None None 0.26 6 Recovered | 6 H. influenzae (89.1%)
(Patients with H. influenzae identified by rapid metagenomics and qPCR)
60 | F | B Ward AEBX None CAZ 0.02 2 Recovered | 7 H. influenzae (98.1%) Early de-escalation would be achieved if the clinicians knew Meta.
60 | M | B RICU AECOPD II Chronic pulmonary heart disease | RF PIP/TAZ+LVF 0.09 1.66 Recovered | 12 H. influenzae (80.6%)
68 | M | S Ward AEBX Diabetes | None CAZ 0.26 7 Recovered | 8 H. influenzae (20.8%)
81 | F | S Ward CAP Chronic bronchitis; PAH; Chronic cardiac insufficiency | Pleural effusion MFX | CAZ 0.21 3 Recovered | 16 H. influenzae (29.4%); L. rhamnosus (15.1%)
38 | M | S Ward AEBX Lung transplant; Emphysema | RF PIP/TAZ 1.77 59 Recovered | 7 C. striatum (48.9%); H. influenzae (20.1%);
S. anginosus(15.6%)
43 | F | S SICU HAP
(late-onset)
None | Brainstem hemorrhage CRO | PIP/TAZ; CAZ 2.55 92.62 Recovered | 27 H. influenzae (64.4%); P. aeruginosa (16.4%) H. influenzae and P. aeruginosa co-infection confirmed by both clinical diagnosis and Meta.
90 | M | S Ward AECOPD I TB | None CAZ | ETP; PIP/TAZ 13.8 53 Recovered | 13 H. influenzae (37.5%); P. aeruginosa (12.5%)
60 | F | S Ward AEBX AECOPD; PAH; Diabetes; Sjögren’s syndrome | None None 0.3 5 Recovered | 11 H. influenzae (56.0%); P. aeruginosa (32.7%);
R. mucilaginosa (5.7%)
65 | M | B Ward CAP-ICH Esophageal cancer; Chemotherapy | Myelosuppression PIP/TAZ+TMP 0.23 5.41 Recovered | 9 P. aeruginosa (85.7%); H. influenzae (4.5%);
S. anginosus (2.8%)
(Patients with M. catarrhalis identified by rapid metagenomics and culture)
56 | M | B RICU AEBX Chronic pulmonary heart disease | RF CIP+CAZ | CIP; CAZ; AZM 0.55 109.56 Recovered | 19 M. catarrhalis (97.4%) M. catarrhalis in AEBX and AECOPD rapidly identified by Meta.
72 | M | S Ward AECOPD II Chronic bronchitis | None None / 13 Recovered | 4 M. catarrhalis (92.4%); R. mucilaginosa (1.7%)
54 | F | S Ward AECOPD II None None / 7 Recovered | 17 S. parasanguinis (20.3%); M. catarrhalis(18.4%); V. atypica (13.0%)
67 | M | S Ward AECOPD II Chronic pulmonary heart disease | None None / 8 Recovered | 6 P. aeruginosa (69.1%); M. catarrhalis (27.3%)
(Patients with M. catarrhalis identified by rapid metagenomics and qPCR)
54 | M | B RICU SCAP GRED | RF; Sepsis; Pleural effusion PIP/TAZ | IPM; VCM 0.02 2 Dead | 5 M. catarrhalis (18.4%) De-escalation was not achieved because the clinicians did not know the causative pathogens.
87 | M | S Ward AECOPD II GRED; Cardiac insufficiency | None ZOX 0.29 22 Recovered | 14 M. catarrhalis (87.9%); R. mucilaginosa (9.4%)
62 | M | B RICU CAP-ICH Rheumatoid arthritis; Rheumatoid lung disease; Diabetes; GRED; Cardiac insufficiency | None ZOX+LVF 0.05 96.19 Recovered | 6 M. catarrhalis (24.0%); V. atypica (10.2%);
R. mucilaginosa (8.4%)
72 | M | S Ward AECOPD I Bronchiectasis | RF CSL+LVF 53 53 Recovered | 5 M. catarrhalis (88.8%); R. mucilaginosa (1.2%)
58 | M | B Ward AECOPD II Pulmonary interstitial fibrosis; Diabetes | None MFX+CSL 0.21 3.41 Recovered | 13 M. catarrhalis (12.5%)
a

Gender: M, Male; F, Female. Sample type: S, Sputum; B, Bronchoalveolar lavage fluid.

b

RICU, Respiratory intensive care unit; SICU, Surgical intensive care unit

c

CAP, community acquired pneumonia; SCAP, severe community acquired pneumonia; HAP, hospital acquired pneumonia; AECOPD, acute exacerbation of chronic obstructive pulmonary disease, AnthonisenⅠ/Ⅱ; AEBX, Acute exacerbation of bronchiectasis

d

CTEPH, chronic thromboembolic pulmonary hypertension; NS, Nephrotic syndrome; GERD, Gastroesophageal reflux disease; PAH, pulmonary arterial hypertension; TB, Tuberculosis; ILD, Interstitial lung disease; RF, Respiratory failure; NHL, non-Hodgkin’s lymphoma.

e

If the antibiotics are not changed, only empirical therapy was reported.

AMC, Amoxicillin/clavulanic acid; AZM, Azithromycin; AMK, Amikacin; CAZ, Ceftazidime; CSL,Cefperazone/Sulbactam; CRO, Ceftriaxone; CIP, Ciprofloxacin; ETP, Ertapenem; FOX, Cefoxitin;

INH,Isoniazide; IPM, Imipenem; LVF, Levofloxacin; MFX, Moxifloxacin; MOX, Latamoxef; MEPM, Meropenem; ORN, Ornidazole; PIP/TAZ, Piperacillin/tazobactam; TGC, Tigecycline; TMP, Trimethoprim; VCM, Vancomycin; ZOX, Ceftizoxime.

f

PCT, Procalcitonin.

g

CRP, C-reactive protein.

h

Length of stay.

i

Meta, rapid metagenomics or identification results of rapid metagenomic.