FIG. 3.

BAX expression in yeast results in hyperpolarization of mitochondria. (A and B) YM1372 yeast cells harboring GAL vector (Vector) or GAL-BAX (BAX) were washed in water and resuspended in SG medium. After 24 h of growth in SG medium, the cells were diluted in SG medium to an optical density at 600 nm of 0.1, incubated with 40 nM DiOC₆ (left) or 2 μM Rh123 (right) for 10 min at 30°C, and analyzed by cytofluorometry. A shift of the DiOC₆ or Rh123 peaks to the right indicates an increase in Δψ_m. (C and D) YM1372 yeast cells harboring GAL vector (C) or GAL-BAX (D) were grown as in panel A. After 24 h of growth in SG medium, the cells were incubated with DiOC₆ in the absence or presence of CCCP for 10 min at 30°C and analyzed by cytofluorometry.