FIG. 4.

BAX expression in a variety of genetic backgrounds lacking individual components of mitochondria results in growth arrest. (A) Expression of BAX in wild-type and mutant yeast cells. Wild-type or mutant yeast cells harboring GAL-HA-BAX were washed in water and resuspended in SG medium. Total-cell extracts were prepared after 16 h of growth in SG medium, and Western blots were developed with HA.11 MAb (Babco) directed to the HA epitope. Each lane was loaded with 20 μg of protein. (B) Yeast cells lacking individual components of mitochondria and harboring GAL-BAX fail to grow on galactose-based plates. Cells were cotransformed with plasmids as in Fig. 1B. Only the lower halves of the galactose-based plates are shown. (C) Comparison of BAX/empty-vector viability ratios of the wild-type (W.T.) and mutant strains shown in Table 1.