FIG. 5.
BAX expression in a variety of genetic backgrounds lacking individual components of mitochondria results in changes in mitochondrial membrane potential and ROS production. (A) Wild-type or mutant yeast cells harboring GAL vector (Vector) (left panel). GAL-BAX (BAX) (left and right panels), or GAL-BAX together with ADH-BCL-XL (BAX/BCL-XL) (right panel) were washed in water and resuspended in SG medium. After 24 h of growth in SG medium, the cells were diluted in SG medium to an optical density at 600 nm of 0.1, incubated with 40 nM DiOC6 for 10 min at 30°C, and analyzed by cytofluorometry. (B) The experimental procedure was identical to that in panel A, except that cells were incubated with 2 μM hydroethidine. Hydroethidine converted to ethidium (HE to Eth) reflects the production of ROS.