TABLE 1.
Survival of yeast strains plated on glucose after 24 h of induction of BAXa
| Strain | Viability of cells containingb:
|
BAX/vector ratio | |
|---|---|---|---|
| Vector (n) | BAX (n) | ||
| Wild type | 0.65 ± 0.01 (3) | 0.002 ± 0.00001* (3) | 0.003 |
| Δatp2 | 0.12 ± 0.01 (3) | 0.11 ± 0.001 (3) | 0.92 |
| Δatp4 | 0.400 ± 0.001 (3) | 0.175 ± 0.001* (3) | 0.44 |
| Δcyc1 Δcyc7 | 0.629 ± 0.0001 (3) | 0.326 ± 0.003* (3) | 0.51 |
| Δcyp3 | 0.74 ± 0.01 (3) | 0.12 ± 0.0001* (3) | 0.16 |
| Δpor1 Δpor2 | 0.96 ± 0.01 (3) | 0.17 ± 0.0007* (3) | 0.18 |
| [rho0] | 0.39 ± 0.006 (6) | 0.47 ± 0.003 (6) | 1.20 |
Cells of the indicated genotypes containing GAL-BAX or control vectors were grown in galactose-containing medium for 24 h in a diluted, and appropriate cell numbers (as determined by Coulter counter analysis) were plated onto plates containing glucose.
The numbers of cells able to form viable colonies in each case were determined after 2 days of growth at 30°C and compared to those expected. Standard deviations are indicated. n indicates numbers of cultures tested. ∗, significantly different from vector.