FIGURE 4.

Calycosin inhibits NLRP3 inflammasome activation in lung tissue. The ligated or LPS-injected mice were treated with calycosin by gavage at 0 and 6 h. After 12 h, NLRP3 inflammasome associated protein levels in lung tissue were determined by western blot analysis. The quantitative densitometry of the protein expressions was determined by Image J software. Densitometric values were normalized to β-actin. The caspase 1 activity in lung tissue was determined by a commercial kit. Data are presented as means ± SEM. *p < 0.05; **p < 0.01 vs. the CLP or LPS group.