Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 19;12:690549. doi: 10.3389/fphar.2021.690549

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Xia, Cao, Sun, Hong, Tang, Yu, Hu, Ma, Qin and Bao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Calycosin inhibits LPS-induced NLRP3 inflammasome activation in BMDMs. BMDMs cells were treated with the different concentrations of calycosin in the presence of 500 ng/ml LPS and 5 mM ATP for 24 h. (A) The levels of IL-1β and IL-18 in the supernatant were determined by ELISA assay. (B) The protein levels of NRLP3 inflammasome were determined by western blot. The quantitative densitometry of the protein expressions was determined by Image J software. Densitometric values were normalized to β-actin. (C) The effect of CA on the interaction among NLRP3, ASC, and caspase 1 were determined by co-immunoprecipitation assay. (D) The caspase 1 activity in BMDMs was determined by a commercial kit. Data are presented as means ± SEM. *p < 0.05; **p < 0.01 vs. the LPS+ATP group.