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. 2021 Oct 19;12:745786. doi: 10.3389/fgene.2021.745786

FIGURE 6.

FIGURE 6

Inflammatory cytokines and chemokines in the bone marrow niche. (A) Quantitation of inflammatory cytokines and chemokines in young and aged bone marrow niches via a Cytoplex Assay. Data are presented as the mean ± SEM.*p < 0.1; **p < 0.01; ***p < 0.001. (B) Ganulocyte/macrophage (GM) and B cell colony formation was measured for young or aged HSPCs incubated for 10 days in methylcellulose in the presence of either DMSO vehicle or IL-1β (25 ng/ml), TNF-α (1 μg/ml), AS101 (2 μg/ml, IL-1β inhibitor) and R7050 (2 μg/ml, TNF-α receptor antagonist). Graph shows mean ± SEM. (C) Dot plots showing the differences in the contribution to different inflammatory signaling pathways. Upregulated cytokines and chemokines are marked in red and downregulated cytokines and chemokines are marked in blue. The size of the dots represents the change in contribution. (D) Circle plot of the cell-cell communication network showing that the inflammatory pathway network (IL2, CCL and complement signaling pathways) became more complex and interconnected in the aged bone marrow niche.